Blood Group A & Forssman Antigen antibody

Catalog No. ABIN6151248

This Mouse Monoclonal antibody specifically detects in IF, IHC (f) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Blood Group A & Forssman Antigen antibody (ABIN6151248)

Target: Blood Group A & Forssman Antigen

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HE-193

Product Details

Purpose: Mouse Monoclonal anti-A, Forssman (HE-193), Purified, BSA-free

Characteristics: This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A

Isotype: IgM kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

KG1 cells or human colorectal carcinoma tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details

Target: Blood Group A & Forssman Antigen

Alternative Name: A, Forssman

Molecular Weight: Multiple

Gene ID: 28, 654423

UniProt: P16442